Speed and enhanced PCR optimization functions like the 2D-Gradient make the Mastercycler X50 the ideal PCR cycler for advanced research in molecular biology. The excellent block temperature regulation gives rise to the next stage of PCR reproducibility, whereas the adaptable user management and profound documentation capabilities give peace of mind to laboratories working to set standards.
A highly intuitive touch display, low noise levels, low power consumption, and the versatile flexlid® concept complete the product to be a powerful PCR cycler. Up to 10 PCR cyclers can be combined – ideal for high throughput applications or labs with a high number of users running different assays. Should you feel you need more flexibility or throughput, up to 50 PCR cyclers can be combined in a computer-controlled network.
PCR Optimization of β-actin Gene with 2D-Gradient technique.
2D-Gradient allows you to optimize both the denaturation temperature (bottom to top) and the annealing temperature (left to right) during the same run. Higher denaturation temperatures can have the advantage of increased specificity while lower denaturation temperatures reduce stress on biomolecules and can lead to increased yield. Assays that struggle to work reliably at a 95° denaturation temperature could benefit significantly from the optimization of the denaturation temperature.
PCR should be faster. That gives you quicker results, throughout the day, and a faster transition to optimal temperatures. The Mastercycler X50 heats with 10 °C/s and cools with 5 °C/s. Make the speed of the Mastercycler X50 your research advantage.
There ia a large variety of cyclers available with different ramp rates published.
If you compare the corresponding run times by performing the same protocol using different cyclers, you see that ramp rates do not tell the full story about whether a cycler is fast or not. For more details, see Application Note 274.