Eppendorf Tubes are the benchmark for simple and safe sample preparation from 0.5 to 2.0 mL. For larger volumes, 15 mL and 50 mL conical tubes are available. With medium sample volumes you may face an issue: they need to be processed with large conical screw cap tubes - impractical, inconvenient and often prone to contamination. The availability of snap cap and screw cap formats enable the optimized choice for different requirements in sample preparation, incubation, storage, or other applications.
Not all consumables are created equal!
Substances which are released from plastic consumables (leachables) may have an influence on laboratory experiments. When they absorb light in the UV range they can disrupt DNA and protein quantifications. The Eppendorf Tube 5.0 mL is manufactured without the use of slip agents, plasticizers and biocides --substances that have been shown to affect bio–assays and laboratory measurements-- and thus offers a safe alternative for sample preparation and storage. Graph: Shown are UV absorbance spectra of pure water incubated (30 min. 90 °C) in tubes from different manufacturers. Data shown in the diagram were generated with 5.0 mL snap cap format - for additional information and protocol description please see Application Note 264.
The 5.0 mL system
50 years ago Eppendorf launched the microliter system consisting of 1.5 ml tubes, Thermomixer, microcentrifuge and Eppendorf pipettes. With the same concept in mind we developed the 5.0 mL system. To make your lab routines faster and easier, we are offering the system components to go hand–in–hand with the Eppendorf Tube 5.0 mL:
› Eppendorf Tubes 5.0 mL
› Tube rack and storage box
› Thermoblocks for Eppendorf Thermomixer
› 5 mL pipettes (manual and electronic) and epTIPS - High–speed rotors for Eppendorf centrifuges
› A range of universal centrifuge adapters for use in existing rotors
› Adapters for use in epMotion automated workstation
› Easier processing of samples up to 5.0 mL;
› Higher yields in DNA isolation, especially with samples with limited quantity of source material (see Application Notes 262 and 263)
› Convenient and safe cell culture applications
› Easier sample access lowers the risk of contamination
› Preparation of mastermixes and buffers
› Less storage space needed
› Safe cell and tissue lysis Graph: Higher yields in DNA isolation–plasmid DNA yields can be significantly increased with low–copy plasmids by increasing the culture volume to 5 mL. The Eppendorf Tube 5.0 mL allows to conduct DNA purification safely and contamination–free, without dividing samples among several smaller tubes.
Data shown in the diagram were generated with 5.0 mL snap cap format - for additional information and protocol description please see Application Note 262.